Location: Sugarcane Production Research
2012 Annual Report
Solexa sequence data will be generated by the cooperator from DNA/RNA bulked from genotypes selected by ARS and segregating for resistance to brown and orange rust of sugarcane as well as their respective parents.
Criteria used by ARS to select the populations will be as follows: High quality and accurate and Replicated spatially and/or over time.
The Cooperator will evaluate Solexa sequencing for marker discovery, mapping and marker trait association using sugar cane material and phenotypic data.
Brown rust reactions were determined in a population resulting from the cross, Green German X Ind 81-146. Parental clones and bulks were analyzed and sequenced to develop potential molecular markers (non-Bru1) for brown rust resistant genes. Potential markers are being evaluated.