2011 Annual Report
Solexa sequence data will be generated by the cooperator from DNA/RNA bulked from genotypes selected by ARS and segregating for resistance to brown and orange rust of sugarcane as well as their respective parents.
Criteria used by ARS to select the populations will be as follows: High quality and accurate and Replicated spatially and/or over time.
The Cooperator will evaluate Solexa sequencing for marker discovery, mapping and marker trait association using sugar cane material and phenotypic data.
Hybrid sugarcane clones showing resistance or susceptibility to brown rust of sugarcane caused by the pathogen Puccinia melanocephala were identified from a wide cross mapping population that is maintained in replicated plots at Canal Point. Brown rust susceptibility data was examined from multiple years of experiments. Leaf tissue from resistant and susceptible clones and the two respective parents were sampled according to protocols supplied and mailed to Syngenta.
Progress was monitored via site visits, emails and phone calls.