EVALUATION OF HLB/ACP RESISTANCE IN PONCIRUS TRIFOLIATA HYBRIDS AND ASSESSMENT OF DNA MARKERS AND POSSIBLE RESISTANCE GENES
Subtropical Insects and Horticulture Research
2011 Annual Report
1a.Objectives (from AD-416)
Evaluate array of Poncirus hybrids and standard cultivars to ultimately identify which genes are associated for use in conventional and transgenic plant improvement.
1b.Approach (from AD-416)
Plant replicated trial with 8 plants of each of numerous citranges (from a well-characterized mapping population), other poncirus hybrids and standard cultivars. Material will be at USDA/ARS Picos Farm where HLB and ACP are rampant and will be screened in response to natural exposure.
This project is related to Objective 1: Create new genetic combinations of citrus; Objective 2: Screen germplasm for important traits and select superior individuals; and Objective 3: Evaluate selections for field performance and other traits.
More than 120 citranges, from a well-characterized mapping population, and other trifoliate hybrids (plus sweet orange standards) have been propagated for a replicated trial, will be planted in July 2011, and monitored for Liberibacter development and huanglongbing (HLB) symptoms. Data from this trial should provide information on markers and perhaps genes associated with HLB resistance, for use in transgenic and conventional breeding.
Progress was shared with the cooperator through email, phone conversations and in meetings.