Location: Foreign Animal Disease Research
2013 Annual Report
1. We have expanded on our analysis of bovine lymphyocyte (BoLA) Class II expression in Holstein cattle. We established a method for determining gene alleles of cattle, which allows us to infer the MHC class II haplotype and determined accuracy. We have typed over 120 cattle and identified 4 dominant BoLA class II haplotypes which are present in 92% of animals tested. Using these methods, we determined the MHC class II haplotypes for 22 cattle. The three most common haplotypes found in the herd were chosen for tetramer production under objective 2. Further patterns of allelic expression are being identified in individual animals contributing to our understanding of class II gene expression which allows us to infer the MHC class II haplotype and the potential relationship between class I and class II expression.
In addition in FY 2013, 2 molecules called DR and DQ, from Bovine haplotype 24a, have been identified for synthesis. One class II protein has been synthesized. Sequencing of the remaining proteins is underway. A total of 8 molecules are in production that will allow for 6 tetramers to be made. Assays to quantify peptide-tetramer binding affinity have been successfully developed for human MHC class II proteins at our collaborators lab at the University of Copenhagen and we demonstrated these assays work for bovine proteins as well. This technology is now being transferred to UVM.
No technologies were transferred to other entities and no publications were written during this fiscal year.