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United States Department of Agriculture

Agricultural Research Service

Research Project: Proof of Concept Testing of Pvy Resistance Exploiting Factor 4e(eif4e) over-Expression: Targeting Key Varieties in the Industry

Location: Small Grains and Potato Germplasm Research

Project Number: 5366-21000-032-04
Project Type: Specific Cooperative Agreement

Start Date: Sep 14, 2011
End Date: May 10, 2016

Objective:
Develop Potato Virus Y (PVY) resistance in existing cultivars by: 1)testing the S. hirsutum pot-1 gene as a source of PVY resistance in potato; 2) doing a molecular characterization of eIF4E-transgenic lines; and 3) produce minitubers for proof of concept field studies.

Approach:
Inoculation studies will be done using Potato Virus Y O strain (PVYO) Potato virus Y NTN strain (PVYNTN), and Potato virus Y N:O strain (PVYN:O) strains in a greenhouse to assay the resistance in four cultivars transformed with the pot-1 gene. Molecular characterization of the transgenic lines will be assayed for sequence insertion and copy number using a Southern Blot analysis. Minitubers of the transgenic lines will be produced in sufficient numbers to allow replicated field trials for PVY resistance in 2012.

Last Modified: 8/21/2014
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