Start Date: Oct 01, 2010
End Date: Sep 30, 2011
New pathogens of invasive weeds in the U.S. and Turkey will be collected each year of this agreement. These pathogens will be isolated from diseased tissue either at FDWSRU or OMU or both. Studies on inoculation and infection efficacy of pathogens collected in Turkey in the past are currently ongoing in the containment laboratory at FDWSRU. Newly collected pathogens under this agreement will be studied in a like manner. Results of these studies will enable development of inoculation protocols that give maximum infection and disease development. Discovery of the best inoculation procedure and timing of inoculation to produce infected plants will enable instigation of epidemics in the field in Turkey. These protocols will be used for infection and host range studies in the field with the indigenous pathogen isolates from Turkey. These studies will be carried out simultaneously with inoculation and infection experiments. Inoculum of pathogens collected in Turkey will be initially increased by our collaborator at OMU in Samsun, Turkey. The collaborator will inoculate host weeds to further increase inoculum for field tests. The collaborator will also locate fields infested with host weeds that can be used for our field tests. FDWSRU will supply the collaborator with seeds of some non-target test plants and seeds of other non-target test plants will be collected from Turkey. Inoculation, monitoring, harvest, reisolation, and propagation of plants in and around OMU will not be problematic. Good facilities at OMU laboratories will help in accomplishing these activities. No hazardous procedures, materials, situations, or activities will be encountered. In short, successful infection, host range, epidemiology, and pathogen-spread studies in the native range of both the host and the pathogen are entirely feasible. Multiple successes, with different pathogens and hosts, within the anticipated three-year duration of the proposed project is very likely.