1a.Objectives (from AD-416):
Develop Potato Virus Y (PVY) and nematode resistance by. 1)screening advanced breeding clones and released varieties with molecular markers for Potato Virus X (PVX) and Potato Cyst Nematode (PCN) (Globodera pallida);. 2)bioassay characterization of resistant clones/varieties for PVX, PVY, and PCN;. 3) make crosses from resistant parents.
1b.Approach (from AD-416):
Multiple entries from North America potato breeding programs will be screened for PVX and PCN resistance using molecular markers and bioassay data. Bioassays of putative PVX, PVY, and PCN resistant clones will be done at the USDA, Agriculture Research Service (ARS) in Aberdeen, Idaho, for PVX and PVY, and at Moscow, University of Idaho for PCN resistance. Resistant clones/varieties will be used in a crossing block to generate a virus and nematode resistant population for further screenings/evaluation.
A population of 88 full-sibling progeny was obtained from the cross of GemStar Russet and OR00054-1. Initial results show that OR00054-1 carries the PCN resistant gene GpaIVadg. Of the 88 progenies, 23% showed four resistance genes (PVYadg, Gpa2, PVX Nb and PVX Rx1) while 33% showed PVYadg and PVX Rx1 resistance genes. Bioassay tests to confirm PCN resistance are still to be done. A subset of 40 clones is currently being tested for PVX resistance in the field in Idaho. These clones were planted in a replicated field plot and inoculated with PVX. At harvest, tubers will be collected from the field and after sprouting will be grown out in the greenhouse. Samples will be taken from the resulting plants and lab tested for the presence of PVX. Resistant rankings will be obtained from the results. The research conducted contributes to all four research objectives of the in-house project.