Location: Food and Feed Safety Research
Project Number: 6435-41420-006-01
Start Date: Sep 15, 2010
End Date: Sep 14, 2015
The present proposal is centered upon previously isolated Apergillus (A.) parasiticus sec- (for secondary metabolism minus) strains, that display altered morphology and sporulation. Recent work from our laboratory has shown that a mutation in aflR coding or promoter region is not responsible for the sec-phenotype. Yet, the aflR expression is lowered in the sec-strains. There is no expression of the aflatoxin pathway genes, and aflR overexpression does not reverse the sec- phenotype. Preliminary results have also revealed clear differences between the sec- and their parental sec+ (for secondary metabolism plus) total protein-profiles. Proteomics and microarray technology will be used to determine the differences between sec+ and sec- strains at the molecular level. These differences will provide insights into the global regulatory mechanisms governing aflatoxin synthesis.