2011 Annual Report
1a.Objectives (from AD-416)
1: To determine the ethylene biosynthetic capacity of the tomato plant. [NP 301, C 4, PS 4A]
2: To determine some of the molecular details of the regulation of the tomato genes by the hormone auxin. [NP 301, C 4, PS 4A]
1b.Approach (from AD-416)
Ethylene is a unique plant signaling molecule. The hydrocarbon gas, known as the fruit ripening hormone, controls many aspects of plant growth and development, and its synthesis is highly regulated. Recent experimental evidence indicates that the key enzyme in the ethylene biosynthetic pathway,1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) is encoded by a highly divergent multigene family in various mono and dicotyledonous plants. The members of each family are differentially regulated by various inducers and developmental stages. Studies on the heterodimerization properties of the various ACS isozymes are proposed in order to determine the biochemical capacity of the ACC gene family. Functional intermolecular complementation and biomolecular fluorescence complementation (BiFC) experiments will be carried to achieve this objective. Furthermore, molecular experiments are proposed to determine which of the Auxin Response Factor (ARF) gene family members regulates the various ACS genes in tomato. REPLACES 5335-21430-006-00D (9/10).
The scientist retired and a new scientist will start in the next fiscal year (November 2011). A new project plan will be written at that time. A Specific Cooperative Agreement (SCA), 5335-21000-032-01S, entitled "Agricultural Crop Improvement through Genomic and Molecular Research on Mechanisms of Plant Growth and Adaption to a Changing Environment" between the Plant Gene Expression Center and UC Berkeley was used to support research in plant molecular genetics. In addition, the SCA will be used to develop the infrastructure needed for the new scientist’s research program.