1a.Objectives (from AD-416):
Obtain the necessary data for foreign regulatory packages for the plum pox virus resistant transgenic Prunus domestica cv. 'HoneySweet', including sequencing the 'HoneySweet' genome, identifying the location and structure of all transgene insertion events, and conducting fruit compositional studies.
1b.Approach (from AD-416):
The 'HoneySweet' plum genome will be sequenced. Assembly will be performed using the finished peach genome as a template. Transgene insertion events will be identified via BLAST. Existing Prunus reference markers will be tested on 'HoneySweet' to refine the assembled physical map and determine the chromosome locations of transgene insertion events. Fruit compositional studies will be conducted and compared with control non-genetically engineered plums.
Plums have been collected from 10 countries in Europe (under APHIS permits) and have been prepared for compositional analyses to be conducted by a third party certified laboratory in order to evaluate the nutrient composition of genetically engineered and non-genetically engineered plums.