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United States Department of Agriculture

Agricultural Research Service

Research Project: Osec Biotech Transfer

Location: Appalachian Fruit Research Laboratory: Innovative Fruit Production, Improvement and Protection

Project Number: 1931-21000-021-00
Project Type: Other

Start Date: Mar 18, 2010
End Date: Sep 30, 2011

Objective:
Obtain the necessary data for foreign regulatory packages for the plum pox virus resistant transgenic Prunus domestica cv. 'HoneySweet', including sequencing the 'HoneySweet' genome, identifying the location and structure of all transgene insertion events, and conducting fruit compositional studies.

Approach:
The 'HoneySweet' plum genome will be sequenced. Assembly will be performed using the finished peach genome as a template. Transgene insertion events will be identified via BLAST. Existing Prunus reference markers will be tested on 'HoneySweet' to refine the assembled physical map and determine the chromosome locations of transgene insertion events. Fruit compositional studies will be conducted and compared with control non-genetically engineered plums.

Last Modified: 8/1/2014
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