2011 Annual Report
During the past year, a project manager was hired and working with the ARS collaborator a comprehensive plan with Pakistani partners is being developed. The present goal of the proposed three year program is to identify sources of resistance to CLCuV and transfer them into adapted lines. Cotton germplasm will be evaluated for CLCuV resistance; genetic mapping populations developed to identify DNA markers to assist in the transfer of the resistance to adapted breeding lines, and methods will be developed to monitor changes in CLCuV virulence and occurrence in Pakistan cotton production regions. As a secondary method of possible control, transgenic methods to combat the virus will be investigated.
Listing of Pakistani partner institutes with a short responsibility descriptor is provided: Virus screening - National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad; Punjab University, Lahore. Location for holding and multiplying germplasm in Pakistan for seed sent from U.S.A. germplasm collection - Central Cotton Research Institute Sakrand, Sakrand. Testing of U.S.A. germplasm collection for CLCuV resistance - Cotton Research Station, Vehari; NIBGE; Central Cotton Research Institute, Multan. Small farmers agronomic practices to reduce CLCuV pressure - National Agricultural Research Center, Islamabad; Punjab Extension Service. Transgenic approaches for resistance - NIBGE; Centre of Excellence in Molecular Biology, Lahore. Research activities during this reporting period were monitored by the ADODR through email communications with the cooperator, phone conversations, Skype calls and through in-person discussions with the cooperator in Pakistan and Syria.