1a.Objectives (from AD-416)
Collaborate with ARS researchers to provide germplasm for Cotton Leaf Curl Virus (CLCuV) screening and transfer resistance identified into adapted cotton lines. Cooperative work with DREC will ensure timely production and transfer of seeds for Cotton Leaf Curl Virus (CLCuV) screening in Pakistan. Lines identified with resistance can be crossed to adapted cotton lines and the progeny evaluated quickly and efficiently. Resistant and susceptible lines of the same species can be crossed to develop mapping populations.
1b.Approach (from AD-416)
DREC will increase some of the germplasm accessions from the Cotton Germplasm Collection, and process seed for Cotton Leaf Curl Virus (CLCuV) screening. Putative resistant lines will be used as parents in crosses to transfer resistance to cotton lines adapted to U.S. cotton growing regions. Markers for resistance will be tracked in progeny of crosses involving parents resistant to CLCuV to confirm transfer of the resistance genes, and progeny will be evaluated under field and greenhouse conditions for suitable agronomic properties and fiber quality.
Seed for Cotton Leaf Curl Virus (CLCuV) field screening in Pakistan was grown at the winter nursery in Tecoman, Mexico, and at Stoneville, MS. This project covers the Stoneville material grown for the winter nursery and for seed increase and evaluation of additional material to be sent to Pakistan to enrich their in situ germplasm collection. Land was set aside for the seed increases and labor secured to assist with plot planting, maintenance and harvest. The material was planted the first week of May 2011. The lines are currently being evaluated for morphological and agronomic traits and will be harvested in late September and early October. Research activities during this reporting period were monitored by the ADODR through email communications with the cooperator and through in-person discussions with the cooperator.