Identification and Introgression of Cotton Leaf Curl Virus Resistance into Cultivated Cotton
Genomics and Bioinformatics Research Unit
2012 Annual Report
1a.Objectives (from AD-416):
Collaborate with ARS researchers to provide germplasm for Cotton Leaf Curl Virus (CLCuV) screening and transfer resistance identified into adapted cotton lines. Cooperative work with DREC will ensure timely production and transfer of seeds for Cotton Leaf Curl Virus (CLCuV) screening in Pakistan. Lines identified with resistance can be crossed to adapted cotton lines and the progeny evaluated quickly and efficiently. Resistant and susceptible lines of the same species can be crossed to develop mapping populations.
1b.Approach (from AD-416):
DREC will increase some of the germplasm accessions from the Cotton Germplasm Collection, and process seed for Cotton Leaf Curl Virus (CLCuV) screening. Putative resistant lines will be used as parents in crosses to transfer resistance to cotton lines adapted to U.S. cotton growing regions. Markers for resistance will be tracked in progeny of crosses involving parents resistant to CLCuV to confirm transfer of the resistance genes, and progeny will be evaluated under field and greenhouse conditions for suitable agronomic properties and fiber quality.
Seed from the U.S. Obsolete Variety Collection and a core set of Gossypium (G.) hirsutum from the national cotton collection, were grown at Stoneville, MS, in 2011 and subsequently processed and delinted. In total, 113 lines from the Obsolete Variety Collection and 84 lines from the Core Set were packaged for shipment to Pakistan. These will be screened for Cotton Leaf Curl Virus (CLCuV) in Pakistan in 2012. Import permits and phyto-sanitary certificates were obtained and the seed was shipped to Pakistan in mid May. Land was prepared and additional seed increases are being grown in the summer of 2012.
January 17, 2012, a Research Technician came onboard for the Specific Cooperative Agreement technical support position. The technician processed and packaged the seed for shipment to Pakistan from the Stoneville, MS, grew material as well as all the material grown at the 2011 Cotton Winter Nursery. The technician assisted with plot planting, data collection and plot maintenance. Late April the Research Technician gave notice that he had been accepted to graduate school and left May 30th. His position has been posted on the Mississippi State University job site and the position is on track to be filled by mid August.
The project has reached the point where the assistance of the Research Associate is required and the job description and hiring requirements are being prepared.