1a.Objectives (from AD-416)
Cultivated cotton is particularly susceptible to many different forms of pathogens including viruses and nematodes. Resistance to such pathogens can often be found in germplasm collections, especially those that represent uncultivated lines or related species. Unfortunately cotton seed has limited viability with an average storage time of 15 years. The ARS Cotton Germplasm Collection has limited amounts of these seed sources and many need to be regenerated before intensive screening can occur. To preserve this material and to provide enough seed for screening purposes, extensive seed increases are needed.
1b.Approach (from AD-416)
ARS and its collaborators will transfer selected seed stocks to the Cotton Winter Nursery using established protocols that conform to necessary sanitary requirements. Staff at the Cotton Winter Nursery in Tecoman, Mexico, will plant the seeds and maintain the plants. Flowers from the plants will be selfed and the resulting seed will be processed and sent back to ARS collaborators following established sanitary requirements.
Germplasm material for the project was prepared at ARS College Station, TX, and planted in early October 2010 at the winter nursery in Tecoman, Mexico. Additional labor and supplies were secured to handle this material. The seed material was sent on schedule to the winter nursery and evaluated by ARS partners in January 2011. All material that matured by mid April 2011 was returned for processing. Two shipments were sent to Pakistan and are currently growing in Cotton Leaf Curl Virus (CLCuV) screening plots at multiple locations. Research activities during this reporting period were monitored by the ADODR through email communications with the cooperator and through in-person discussions with the cooperator at professional society meetings.