1a.Objectives (from AD-416):
Investigate and develop attractants for three emerald ash borer parasitoids.
1b.Approach (from AD-416):
Collect, compare and quantify male- and female-specific volatiles. Determine the GC-EAD activity of the sex-specific compounds. Identify sex-specific compounds with biological activity. Synthesize the new compounds and formulate for behavioral testing. Determine the effects of various environmental and biological parameters on the production of sex-specific compounds. Isolate, identify, and synthesize host and tree related attractants. Wind tunnel and field tests.
The emerald ash borer (EAB) is an invasive beetle pest from Asia that is causing widespread mortality of ash trees in the U.S. and Canada. Three species of parasitic wasps originally reared from EAB in China have been released in the U.S. as possible biological EAB control agents. ARS Crop Bioprotection Research Unit scientists at the National Center for Agricultural Utilization Research, Peoria, Illinois, have identified the pheromones of two of these exotic parasitic wasps. In addition, the pheromone of a native parasitic wasp that has adapted to EAB as host has been identified. These male-produced pheromones are attractive in wind tunnel and field cage experiments and attracts both male and female wasps. These pheromones (a blend of several compounds) have been synthesized and are currently being tested in a large-scale field experiments in Michigan and Ohio with the help of APHIS collaborators. So far only male-produced pheromones have been identified with the parasitic EAB wasps. However, we have now identified a pheromone from one of the Chinese species that is only released by female wasps and this pheromone attracts only the conspecific males. These natural attractants could be used in monitoring systems to evaluate the establishment and spread of newly released populations of EAB biocontrol agents, thereby helping forest and woodlot managers in their assessment of EAB biological control.