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United States Department of Agriculture

Agricultural Research Service

Research Project: Pakistan Cotton Productivity Enhancement Program

Location: Genomics and Bioinformatics Research Unit

2011 Annual Report


1a.Objectives (from AD-416)
Mitigate the threat of Cotton Leaf Curl Virus (CLCuV) to the Pakistani cotton crop. Develop resistant germplasm as well as detection and screening methods needed to increase Pakistan’s and the U.S.’s level of preparedness for CLCuV.


1b.Approach (from AD-416)
ARS will work with ICARDA and U.S. universities to implement the collaborative project. Objectives will include: Identify new sources of resistance; transfer resistance genes; develop new tools to identify, characterize, and monitor different strains of CLCuV; develop and test resistant varieties; transfer new techniques, genetic resources, and expertise to Pakistani scientists.


3.Progress Report

The funding for this project goes towards the following projects: 6402-21310-003-11S, Stable Introgression of Cotton Leaf Curl Virus Resistance into Cultivated Cotton and Germplasm Enhancement; 6402-21310-003-10S, Development of Cotton Leaf Curl Virus (CLCuV) Diagnostic Tools and Monitoring of Clcuv; 6402-21310-003-12S, Increase of Cotton Germplasm with Emphasis on Non-Cultivated Species for Examination of Important Disease Resistance Traits; 6402-21310-003-13S, Identification and Introgression of Cotton Leaf Curl Virus Resistance into Cultivated Cotton; 6402-21310-003-14S, Enhancing Cotton Germplasm, Improving Resistance to Cotton Leaf Curl Virus and Supporting Cotton Best Management Practices for Small Farmers, where additional details can be found.

During the past year, a comprehensive international research program was developed to combat Cotton Leaf Curl Virus (CLCuV) by developing resistant cotton lines and studying the virus itself. The CLCuV strain found in Pakistan developed within the country and is the most virulent form known in the world. Germplasm from the USDA ARS Cotton Germplasm Collection is being propagated and sent to Pakistan for screening to see if it carries any resistance. In 2011 over 500 accessions of wild relatives of cultivated cotton were sent to Pakistan and are presently being screened for resistance. The overall goal here is to identify novel forms of resistance, genetically map the resistance to identify a DNA marker associated with resistance, and then integrate the resistant trait into cultivated cotton. Sentential plots were established in Pakistan to trap new isolates of the virus for characterization and to establish protocols for to see if new forms of the virus are evolving. DNA sequencing methods were validated during the year to determine the DNA sequence of numerous isolates of the virus in order to look for changes in the virus. Integrated research programs with Pakistani Institutes and U.S.A. researchers are being established.

Research activities during this reporting period were monitored by the ADODR through email communications with the cooperator and through in-person discussions with the cooperator in Washington D.C. and U.S.A. Embassy in Pakistan, phone conversations, and weekly conference calls.


Last Modified: 11/28/2014
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