1a.Objectives (from AD-416):
Develop immune reagents for veterinary animals of economic importance including cattle, swine, poultry, horses, and aquaculture species.
1b.Approach (from AD-416):
Immune reagents will be developed for basic and applied studies in veterinary animal species. They will be developed into multiplex assays to measure cytokine and chemokine production during infections and host immune responses. As a member of a large consortium grant to develop immunological reagents for five different animal species, we will identify major cytokines and cell surface markers of poultry, express them in suitable expression vectors and validate their function using in vivo and in vitro assay systems.
The main mission of this NIFA-funded AFRI consortium grant is to develop immunological reagents specific for poultry, ruminants, swine, equine and aquaculture species. During this reporting period, we have characterized a protein called CD83 that is primary found on the cell surface of mature chicken dendritic cells. The dendritic cell is an important immune cell type as it can stimulate an immune response from other immune cells, particularly lymphocytes, involved in the development of acquired immunity. To characterize chicken CD83 protein, we developed two distinct mouse antibodies that detect CD83. The antibodies identified other immune cells expressing CD83 and regions in tissues where CD83 positive cells, likely dendritic cells, are found. These antibodies will be useful for basic and applied research on chicken dendritic cells.