1a.Objectives (from AD-416)
To determine how Rumensin affects the bacterial and archaeal populations within the rumen of lactating dairy cows and to determine the effect of this drug on the bacterial populations within dairy wastewater that is routinely used as a fertilizer for crop plants such as tree nuts.
1b.Approach (from AD-416)
In order to determine how different dosages of Rumensin affect the bacterial and archaeal populations within the rumen and waste, rumen fluid and waste samples will be taken from the four dairy cattle receiving either 0, 200, 400 or 600 mg/hd/day. The samples will be assayed by cultural, 16S rRNA gene library analysis and quantitative PCR analysis for total and pathogenic bacteria.
PMR scientists in Albany, CA, have extracted DNA from rumen fluid samples from cattle fed 0, 200, 400 and 600 mg/hd/day monensin and have begun to quantify the levels of pathogenic and nonpathogenic bacteria and protozoa which may be selected for or against by the drug monensin. We have also begun to use molecular techniques to identify the different types of bacteria and protozoa that are present in the wastes produced by these animals to determine if feeding this drug to cattle has an effect on the levels of pathogenic bacteria and protozoa shed in their wastes.
This agreement was established in support of Objective 1 and 2 of the in house project. The goal being to determine if the use of animal manure as a fertilizer can influence the level microbial population structure with specific regard to pathogens on tree nuts.