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United States Department of Agriculture

Agricultural Research Service

Research Project: Effects of Rumensin on Mitigating Eructated, Rumenal, and Fecal Greenhouse Gas (GHG) and Volatile Organic Compounds (VOC)

Location: Foodborne Toxin Detection and Prevention

Project Number: 5325-42000-038-07
Project Type: Reimbursable

Start Date: Jan 01, 2010
End Date: Sep 01, 2012

Objective:
To determine how Rumensin affects the bacterial and archaeal populations within the rumen of lactating dairy cows and to determine the effect of this drug on the bacterial populations within dairy wastewater that is routinely used as a fertilizer for crop plants such as tree nuts.

Approach:
In order to determine how different dosages of Rumensin affect the bacterial and archaeal populations within the rumen and waste, rumen fluid and waste samples will be taken from the four dairy cattle receiving either 0, 200, 400 or 600 mg/hd/day. The samples will be assayed by cultural, 16S rRNA gene library analysis and quantitative PCR analysis for total and pathogenic bacteria.

Last Modified: 9/10/2014
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