1a.Objectives (from AD-416):
Determine the impact of soil management on soil microbial community structure and function using biochemical and molecular methods.
a. soil distubance effects on soil function
b. role of mycorrhiza on P nutrition of corn
1b.Approach (from AD-416):
Biochemical (e.g. lipid profiles) and molecular methods will be used to identify functional groups of soil microorganisms under contrasting management conditions. These measurements will be taken in conjunction with soil process (e.g. nutrient transformation and uptake) and crop production (e.g. yield and nutrient content) measurements.
Greenhouse and field experiments were conducted to assess how nitrogen and phosphorus availability effect the incidence of mycorrhizal infection and subsequent nutrient uptake. Molecular methods were used to determine phosphorus transporter responses to mycorrhizal infection in maize. Lipid profile analysis was completed to compare microbial communities between annual and perennial feedstock systems receiving different nitrogen fertilizer amounts and subjected to different biomass removal treatments.