Location: Food and Feed Safety Research
Project Number: 6435-42000-021-11
Start Date: Sep 01, 2010
End Date: Aug 31, 2015
Identification of novel genes/promoters through differential gene expression analysis in response to Aspergillus (A.) flavus infection such as messenger ribonucleic acid (mRNA) differential display/ subtractive suppression hybridization to complement proteomics/genomic research. Heterologous or native genes/promoters identified through this research or from research on other plant species, such as Louisiana native Smooth Cordgrass (marsh adapted, salt-tolerant), will be employed in transgenic systems to evaluate their efficacy for tolerance to biotic and abiotic stress. Smooth Cordgrass has been shown to have high sequence similarity with corn. Promising gene constructs in transgenic tobacco model system will be advanced for use in transgenic cotton or corn. Finally, transgenic plants will be evaluated for resistance to A. flavus under laboratory, greenhouse and field conditions.