DEEP SEQUENCING OF DIAPHORINA CITRI
Subtropical Insects and Horticulture Research
2011 Annual Report
1a.Objectives (from AD-416)
Produce better annotation of Asian citrus psyllid genome, Diaphorina citri, by producing a deep sequencing run.
1b.Approach (from AD-416)
Using psyllid DNA and RNA, as provided by ARS, conduct a deep sequencing run to aid validation and fill gaps in alignments for psyllid genome.
This project is related to Objective 3b: Develop novel control methods based on disrupting key processes in pest biology.
Psyllid genetic libraries were made for sequencing and shipped to the Los Alamos Lab, Genome Sequencing Center. The libraries will be used to produce a 100 times coverage of the psyllid genome to aid efforts to produce a complete genome sequence. The genome will provide the foundation for emerging strategies towards the genetic management of psyllids.
Progress was monitored through phone calls and email monthly contacts.