Location: Subtropical Horticulture Research
2012 Annual Report
2. To develop 10 to 30 thousand usable Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP) markers to saturate our current genetic linkage maps and to use for association mapping to locate genes involved with tolerance/resistance.
Using next generation sequencing (NGS), thousands of SSR and SNP markers can be easily identified and used for increased map saturation and association genetics thus facilitating QTL identification. We will generate 10 x 454 coverage of the cultivar ‘Hass’ and 50x Illumina coverage for the other three parents of our mapping populations, ‘Bacon’, ‘Simmonds’, and ‘Tonnage’. The identification of 300,000 new SNP markers has been accomplished using this procedure with cacao (Theobroma cacao L.). The SNP markers will be used to develop an Illumina 30K SNP iSelect array.
Molecular markers have been developed for genetic diversity analysis and for the production of molecular genetic linkage maps. Families of avocado, mango, and jackfruit have been produced that should allow the mapping of Quantitative Trait Loci (QTL) involved with disease resistance, fruit quality, and yield. A medium to high-throughput single nucleotide polymorphism (SNP) assay platform was obtained through supplementary funding to genotype the open-pollinated progeny from germplasm accessions that have been planted at the USHRL, Fort Pierce, FL. Laurel wilt has been observed in avocado production orchards in Miami-Dade County within 20 miles of the SHRS station. Open pollinated seed of Mexican accessions has been obtained from University of California, Riverside collaborators for germination and planting at both SHRS and USHRL to identify laurel wilt resistant individuals and to estimate heritability of laurel wilt resistance.