2011 Annual Report 1a.Objectives (from AD-416) The objectives of this cooperative research are to. 1)develop grain quality measurement methods to enable sorting for particular attributes of single seeds using near- and mid- infrared spectroscopy and. 2)develop improved grain storage monitoring methods through the innovative use of carbon dioxide (CO2), relative (RH) humidity and temperature (T) sensing systems. 1b.Approach (from AD-416) For the first objective, grain quality measurement methods using near- and mid-infrared reflectance spectroscopy (NIRS, MIRS) and other non-destructive methods will be developed to segregate single kernels based attributes such as protein, oil and starch. The focus will be to develop accurate and rapid methods (5 -10 kernels/sec) to measure basic seed composition or more subtle characteristics such as oil composition. The second objective will investigate the condition and effects of storage mold development during grain storage. This work will provide a better understanding and interpretation of CO2-RH-T measurements inside commercial grain storage. These systems are currently being introduced as commercial systems but there is a lack of understanding in data interpretation. Iowa State will facilitate trial installations of this equipment at commercial grain facilities. 3.Progress Report Single-seed NIRS calibrations for soybean and corn oil were developed using sample sets from Iowa State University and USDA. Calibration transfer between two single-seed NIRS systems, one at Iowa State and one at USDA-ARS, Manhattan is being studied. Protein calibrations will be developed for these seeds when reference protein is completed. Calibrations will be used to quantify single-seed soybean oil and protein for breeding and genetic studies. These activities were monitored via telephone calls and e-mail conversations to discuss project plans and review program goals and accomplishments.