Discovery and Reconstitution of Cross-Reactive Epitope Targets for H5 and H9 Avian Influenza
Exotic and Emerging Avian Viral Diseases Research Unit
2013 Annual Report
1a.Objectives (from AD-416):
Identify and map highly conserved B-cell epitopes common to surface of H5 and H9 avian influenza (AI) and to test their suitability as homotypic or universal vaccine components.
1b.Approach (from AD-416):
In this study, recombinant epitope mapping will be accomplished using sequence analysis of hemagglutinin genes of H5 and H9 avian influenza virus, and epitopes generated in comprehensive conformer-libraries to be displayed on filamentous bacteriophages. These epitopes will be selected for high evolutionary conservation and designed to elicit cross neutralization against diverse H5 and H9 avian influenza viruses. Most promising H5 and H9 epitopes will be used to immunize chickens and examine for ability to protect from H5 and H9 avian influenza virus challenge, respectively.
This research is directly related to inhouse objective 1 - Characterize variant and emerging avian influenza viruses in live poultry markets and commercial production systems; and inhouse objective 5 - Develop new vaccine platforms designed to control and prevent avian influenza virus outbreaks.
In FY2013, 3 week-old chickens were vaccinated with c300 and scrambled c300s protein with and without montanide adjuvants to produce antisera for neutralizing antibodies. In addition, antisera was produced against other proteins and IgY was extracted and sent to cooperator for additional Western Blot analysis.