Start Date: Oct 01, 2010
End Date: May 30, 2013
Investigate isoform specificity for nitrate reductase (NR) posttranslational modification in vivo, and elucidate the impact of 14-3-3 binding on NR protein degradation. Localize the membrane binding site(s) on sucrose synthase and identify factors that may control the interaction. Use high-resolution spatial and temporal analysis of leaf growth to identify specific areas where leaf growth is occurring. Determine the biochemical factors responsible for the lower activation state of Rubisco, at high temperatures and test potential transgenic amelioration strategies. Further elucidate the role of Rubisco activase in thermal sensitivity/tolerance. Determine the biochemical basis for the "Green Seed Problem" of canola. Perform metabolite analysis of growing leaves under elevated CO2 and O3 to identify key components that may be involved in controlling growth. Determine the factors that lower the activation state of Rubisco under sink- and/or N-limited conditions, which are often encountered when plants are grown under high CO2. Explore the interaction of elevated CO2 with drought on soybean performance. Explore the interaction of elevated CO2 with temperature on soybean and corn performance. Determine if growth at elevated CO2 enhances or ameliorates oxidative stress. Determine the impact of land cover change from row crops to perennial grasses on hydrological cylce and carbon biosequestration.