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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Molecular diagnostics of Phytophthora Spp. from natural ecosystems

Location: Crop Improvement and Protection Research

Project Number: 5305-22000-014-02
Project Type: Reimbursable

Start Date: Aug 01, 2010
End Date: Jun 30, 2013

Objective:
To validate molecular diagnostic tools for identification of Phytophthora ramorum, the pathogen responsible for sudden oak death.

Approach:
Two different molecular diagnostic techniques will be evaluated for their effectiveness in identifying Phytophthora species in infected plat tissue using samples collected from different regions of the USA. One procedure is a TaqMan real time PCR diagnostic marker that exhibits specificity at a genus level (it can identify if a Phytophthora spp. is present) in addition to a species level (different probes can differentiate P. ramorum or P. kernoviae from other species). The second procedure is for determination of mitochondrial haplotype of P. ramorum, which so far has been correlated with the nuclear genotype. The technique uses melt curve analysis to make the determination, which takes much less time than conventional techniques for genotype determinations.

Last Modified: 8/29/2014
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