Project Number: 5350-43000-006-00
Start Date: Jun 24, 2010
End Date: Jun 23, 2015
Research to elucidate genetic regulation of apple fruit texture and aroma will utilize established molecular analytical techniques. All analyses will use resources in place in the research unit. The apple germplasm to be utilized is available via an ongoing collaboration with the Washington State University Apple Breeding Program. This includes reference cultivars as well as a large cross population specifically established for studies related to fruit texture. Metabolomic studies will utilize fruit obtained from commercial and research orchards with chemical analyses conducted using location resources. Chemical analyses (GC- and LC-MS) will be performed using established methods where available with new methods developed as needed. Fruit will be stored using the location’s cold storage and controlled atmosphere facility which consists of 140-2 bushel chambers in which N2, CO2, and air are manipulated to achieve desired proportions in each chamber. Individual chamber temperature control is available for 20 chambers. Field experiments will be primarily conducted in commercial orchards to allow microclimate effects to be evaluated under a range of geographic and horticultural management conditions. Fruit microenvironment prior to harvest will be manipulated using polyethylene bags. Evaluation of fruit epidermis by SEM will utilize equipment in place in the research unit.