Project Number: 6034-41430-005-00
Start Date: Jul 20, 2010
End Date: Jul 13, 2015
Evaluate advanced breeding lines for citrus tomato and small fruits to determine optimal harvest maturity, microbial stability, and overall flavor quality. Evaluate diverse breeding material for citrus, tomato, and strawberry to determine desirable flavor aromas and tastes. Flavor analyses will be done using sensory panels as well as chemical analyses using gas chromatography (GC) mass spectroscopy (MS), GC-olfactometry, and electronic nose for aroma compounds and liquid chromatography (HPLC), HPLC-MS and microplate readers for sugars, acids, vitamin C, carotenoids, phenolic and antioxidant analyses coupled with flame ionization, refractive index and ultraviolet light and photo diode array among other detectors. Sensory panels will consist of consumer or trained descriptive panels to determine preference and fundamental flavor information. Multivariate and other statistics will be used to relate chemical to sensory data. Storage of commodities under study will be done using simulated commercial or abusive conditions for monitoring of quality and decay. Causal decay organisms will be indentified and pre- and postharvest treatments to reduce their population will be tested including pre-harvest deployment of wax-based sprays, sanitizers, and nutrients as well as use of postharvest sanitizers, anti-microbial agents or antagonists with or without coatings. Produce will also be subjected to short-term antimicrobial atmospheres of low oxygen, high carbon dioxide alone or combined with other anti-microbial volatile compounds. Effect of disease (Huanglongbing) on orange juice flavor will also be analyzed and identification and threshold of off-flavor compounds determined.