Start Date: May 25, 2010
End Date: Sep 30, 2010
Exotic pathogens will be collected from symptomatic target weeds in countries where they are native, evaluated for their potential using standard plant pathology methods, and identified using both classical morphological characters and molecular sequence data. Molecular signatures will be developed for unambiguous identification and detection. Pathogens will be evaluated for the risk associated with intended release into ecosystems containing economically and ecologically important North American plant species. Proposals for release of the pathogen into the environment will be developed for review by the Technical Advisory Group for Biological Control Agents of Weeds, and subsequent development of an Environmental Assessment, declaration of Finding Of No Significant Impact and issuance of federal and state permits for release. Inoculum will be prepared in sufficient quantity for release, hosts will be inoculated in the field and provided conditions that favor infection and establishment. Weather and baseline weed population density data will be collected at each release site. The establishment and spread of pathogens will be monitored in the field by recording disease symptoms on the target weed and verifying identification of the pathogen using classical morphology and molecular diagnostic assays. Damage to target weed populations and environmental factors important in pathogen establishment, efficacy and spread, will be measured. Regional collaborations will be established to restore sites where biological control has proven efficacious toward more stable and sustainable ecosystems. BSL recertified on 9-14-09.