Location: Crops Pathology and Genetics Research
Project Number: 5306-21220-004-15
Start Date: Apr 14, 2010
End Date: Dec 31, 2013
A variety of grapevine rootstocks and species will be grown both hydroponically and in potting soil in greenhouses. We will measure the hydraulic conductivity of excised fine roots and whole root systems using ultra-low flowmeters and cell pressure probe technologies and whole root systems using pressure chambers. Functional aquaporin activity will be assessed by measuring reductions in hydraulic conductivity of individual fine roots and whole root systems after treatment with known aquaporin inhibitors. Molecular aquaporin activity will initially be evaluated using aquaporin molecular probes previously developed for grapevines. Over the long term, aquaporin protein abundance and immunolocalization analysis in fine roots will be conducted. Documents Reimbursable Agreement with American Vineyard Foundation. Log 41322.