2013 Annual Report
Independent preliminary research conducted by USDA-ARS in Raleigh, NC, and by the University of Minnesota identified a family of soybean genotypes from Fiskeby, Sweden, that are resistant or partially resistant to drought, iron deficiency chlorosis, toxic soil aluminum, salt, and atmospheric ozone pollution. A population developed from hybridization of Fiskeby III (representing the stress resistance of the Fiskeby soybean family) with Mandarin (Ottawa) (susceptible to drought, ozone, salt, and iron deficiency chlorosis, but resistant to aluminum) will be used to map resistance genes. The original hybridization was made in 2006 and seed of the resulting population were advanced by single seed descent.
Screening the population for iron deficiency chlorosis in Minnesota field plots was completed. Data from 2011 and 2012 are being analyzed in preparation for QTL analysis. Data from both 2011 and 2012 showed significant segregation within the population. Data are being analyzed and formatted for QTL analysis. Programming code for the QTL analyses written for R/QTL to conduct ozone analysis is being adapted for iron deficiency chlorosis.
Weather permitted collection of wilting scores in Minnesota field plots during the summer of 2012, so screening of the population for drought was begun. Wilting scores were collected on two dates (July 5 and July 16) before rain events and confounding differences in maturity prevented further readings. On a scale of “1” (no wilting) to 5 (dead plant), scores for the tolerant Fiskeby III parent ranged from 1-2 and the scores for the sensitive Mandarin (Ottawa) parent ranged from 3-4. The RILs had scores that ranged from 1-4, suggesting that identification of QTL for drought in this population will be possible.
Seed from the early maturing lines in the population were harvested from Minnesota field plots to maintain seeds supplies.