1a.Objectives (from AD-416)
Characterize inheritance of biological nitrogen fixation in dry beans and develop germplasm with improved biological nitrogen fixation.
1b.Approach (from AD-416)
Optimze biological nitrogen fixation in dry beans through genetics and plant breeding. Identify lines with high BNF and cross with lines with low BNF to devlop breeding populations. Evaluate breeding and mapping populations for BNF response and populate with markers for QTL analysis.Documents Reimbursable with IA State. Log 41946.
An array of 25 dry bean genotypes spanning gene pools, mapping populations, and biological nitrogen efficiency as reported in the literature were planted in two locations in Washington. Three treatments were included, no nitrogen and rhizobium, nitrogen only, and rhizobium only to examine biological nitorgen efficiency of the genotypes. Experimental design included 150 four-row plots per location (2 replications x 3 treatements x 25 genotypes) in a strip-block arrangment. Soil samples were collected from each site for nutrient analysis and isolation of endemic rhizobium strains.
This agreement supports Objective 1 of the in-house project, with the goal to Identify, characterize, and tag genes/QTL conditioning traits of economic importance in edible legume production, specifically those affecting biological nitrogen fixation efficiency.
This project was monitored by frequent emails and phone calls with the collaborators.