2011 Annual Report 1a.Objectives (from AD-416) To characterize and compare metabolite profiles in tall fescue endophyte-infected and endophyte-free clone pairs. 1b.Approach (from AD-416) Tall fescue, KY31, containing the wild-type fungal endophyte (Neotyphodium coenophialum) strain has been used to generate endophyte-free lines, constituting of a genetically identical plant background with and without the endophyte. These clone pairs provide a tool to characterize and compare gene expression, metabolite profiles and phenotypic responses, in an identical genetic background, thus allows comparisons based solely on the presence or absence of the endophyte. 3.Progress Report Endophyte-infected (E+) and Endophyte-free (E-) clone pairs have been sent to the Noble Foundation. Clone pairs are being established for experiments. This project was monitored with face-to-face meetings, teleconferences, emails, and involvement in the research.