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United States Department of Agriculture

Agricultural Research Service

Research Project: Metabolite Profiles of Tall Fescue Endophyte-Infected and Endophyte-Free Clone Pairs

Location: Forage-Animal Production Research

2011 Annual Report

1a.Objectives (from AD-416)
To characterize and compare metabolite profiles in tall fescue endophyte-infected and endophyte-free clone pairs.

1b.Approach (from AD-416)
Tall fescue, KY31, containing the wild-type fungal endophyte (Neotyphodium coenophialum) strain has been used to generate endophyte-free lines, constituting of a genetically identical plant background with and without the endophyte. These clone pairs provide a tool to characterize and compare gene expression, metabolite profiles and phenotypic responses, in an identical genetic background, thus allows comparisons based solely on the presence or absence of the endophyte.

3.Progress Report

Endophyte-infected (E+) and Endophyte-free (E-) clone pairs have been sent to the Noble Foundation. Clone pairs are being established for experiments. This project was monitored with face-to-face meetings, teleconferences, emails, and involvement in the research.

Last Modified: 10/22/2014
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