1a.Objectives (from AD-416):
The objective of this cooperative research project is to determine the differences in gene expression between normal and insulin resistant mice fed high fat diets containing processed berry fractions or soluble fibers. Another objective is to determine if differences in transcription by alternative splicing of genes occurs in mice fed identical diets except for differences in fiber solubility.
1b.Approach (from AD-416):
Microarray technology currently allows the analysis of all exons in a gene so that all alternative splicing can be detected. In a pioneering experiment we have identified differences in genes and alternatively spliced genes between normal and insulin resistant obese mice by exon microarray. The expression of these genes must be confirmed by PCR and a pathway analysis of the relationship between these genes and insulin resistance needs to be developed assisted by metabolic pathway analysis software such as GeneSpring.
Progress reported addresses Sub-Objective 1.2 of the parent project: “Assess bioactivity of extracts and residues of 1.1 by feeding mice or hamsters hypercholesterolemic diets and determining physiologic characteristics of obesity related metabolic syndrome”. A feeding study of mice fed grape seed powder and liver and adipose tissues were analyzed using microarray technology that looked at over 1 million gene segments (exons) and a manuscript is in progress.
In Fiscal Year 2013 the expression of genes using a multi-analyte assay for intestinal inflammatory gene expression in mice fed grape seeds. The laboratory work is completed and a manuscript is being prepared.