1a.Objectives (from AD-416):
The long term goal of this project is the development of rapid, reliable, and sensitive diagnostic assays for the identification of insect transmitted viruses, arboviruses, that are suitable for use in veterinary diagnostic laboratories, as well as in the field for use during times of natural or intentionally caused disease epidemics.
1b.Approach (from AD-416):
The ARS and KSU have and are developing assays for detecting genetic material, antigen or antibodies to viruses using a variety of diagnostic formats on a manual laboratory scale. The approach is to evaluate these assays on diagnostic specimens in conjunction with the standard assays used in the veterinary diagnostic laboratory. Those assays that are found to be acceptable then will be further optimized and automated for a high-throughput scale. The primary focus of the proposed studies will be the demonstration and evaluation of new diagnostic assays for arboviruses, followed by the translation of these to validated tests to be used by veterinary diagnosticians.
This project was initiated to develop, expedite the evaluation and standardization of arbovirus diagnostic assays. This project has resulted in technology exchange between ARS and KSU. The ARS multiplex real-time Reverse - Transcriptase Polymerase Chain Reaction genetic amplification assay for bluetongue and epizootic hemorrhagic disease viruses has been transferred to Veterinary Diagnostic Laboratory (VDL) for high-throughput adaptation. The whole-genome amplification and sequencing technology for viruses specifically of the Reoviridae family was transferred to VDL. Currently, streamlining of the Bioinformatics for data assembly and analysis is in progress. A collaborative grant was submitted to acquire a new diagnostic technology that would allow detection of multiple pathogens in a single assay.