2013 Annual Report
1a.Objectives (from AD-416):
To develop knowledge, diagnostic tools, and control practices and technology to address the threat posed to the U.S. Pork Industry by Classical Swine Fever (CSF), African Swine Fever (ASF) and of other diseases of high consequence.
1b.Approach (from AD-416):
Classical Swine Fever (CSF) and African Swine Fever (ASF) are highly infectious diseases of swine. As foreign animal diseases (FADs), an outbreak of CSF, ASF or an unknown threat would significantly affect the U.S. pork industry and export markets. Improved CSF marker live-attenuated vaccines are needed to further reduce the risk posed by this FAD. There are no commercial ASF vaccines, which is a significant gap in the available interventions to stop the spread of ASF. Furthermore, there is a gap in our understanding on the mechanism of ASF pathogenesis. Other needs include improved diagnostic approaches for conducting surveillance. Underlying this research is improved diagnostics and surveillance.
Personnel- During 2012, five individuals have been working on CSF and ASF. A postdoc worked on the cloning and expression of CSFV and pestivirus proteins. A DVM/PhD graduate student has taken over the project. A postdoc is working on the expression of ASF proteins. A graduate student is working on the adaptation of proteins to the Luminex platform.
Classical Swine Fever Virus (CSFV)
The purpose of this project is to express CSFV proteins for serology, including adaptation to Luminex. One consideration is cross-reactivity with BVDV proteins. CSFV Erns full length and fragments were expressed in bacteria. Expression of the full-length E2 protein was not successful. However, fragments of E2 were expressed. ELISA analysis of E2 and Erns proteins against BVDV sera revealed that the E2 protein has the greatest cross-reactivity with BVDV. Therefore Erns is a better target for assay development. Fragments of each protein are being expressed and tested. E2 was cloned into an alphavirus vaccine platform and is being used to generate antibodies in pigs.
African Swine Fever Virus (ASFV) Update:
Work on ASFV has focused on protein expression. Proteins being expressed include p30, p54, and p72. The same genes were cloned into alphavirus as a means to prepare ASFV-specific antibodies in pigs.