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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetics of Rust Pathogen Avirulence and Host Resistance in Hard Winter Wheat

Location: Hard Winter Wheat Genetics Research Unit

Project Number: 5430-21000-010-06
Project Type: Specific Cooperative Agreement

Start Date: Jun 15, 2010
End Date: Jun 14, 2015

Objective:
1) Identify candidate avirulence genes in the wheat leaf rust pathogen, Puccinia triticina, that may condition resistance to wheat leaf rust, 2) Assemble association mapping population in hard winter wheat and analyze for resistance to leaf rust, stripe rust, and stem rust, and 3) Construct and analyze mapping populations for resistance to wheat leaf rust, stripe rust, and stem rust.

Approach:
Candidate avirulence genes will be identified from gene expression studies or by analysis of genomic sequences of Puccinia triticina. Candidates will be tested for ability to induce hypersensitive necrotic responses on resistant wheat varieties. An association mapping population representing diverse hard winter wheat germplasm will be tested in the field and in the greenhouse for resistance to the three rusts. Lines will be genotyped using a large set of SNP markers. Genomic regions associated with lower mean disease scores will be further investigated. Mapping populations will be developed using the doubled haploid (DH) or the recombinant inbred line (RIL) approach for native and exotic sources of resistance to leaf rust, stripe rust, and stem rust.

Last Modified: 9/20/2014
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