2010 Annual Report
1a.Objectives (from AD-416)
1. To identify marker trait association between elite North American oat varieties and key traits including total dietary fiber, beta glucan, vitamin E, crown and stem rust resistance, barley yellow dwarf resistance, height, lodging, plumps, test weight.
2. Develop and use the marker-trait association for predictive assays through the USDA, ARS Genotyping centers by specifically placing the equipment needed to perform the assays.
1b.Approach (from AD-416)
Six-hundred and twelve oat lines representing the diversity important to North American Oat breeding programs and elite lines from each breeding program will be grown in ID, NY, IL, IN, WI, MN, ND, TX, LA, NC, Ottawa, Saskatoon, Winnipeg, and the UK. Agronomic traits will be collected by the breeder in at least ID, NY, Saskatoon, and Winnipeg. Seed harvested from these locations will be test for milling quality for avenanthramide, a-tocotrienol, atocopherol, total dietary fiber, and ß-glucan. Disease data (Crown rust, Stem rust, and BYDV) will be collected in TX, IN, IL, LA, ID, WI, Ottawa, and MN, while freeze tolerance
data will be collected in NC. Genotypic and phenotypic data will be used to determine marker-trait association using JMP Genomics and TASSEL. An open array real-time TaqMan assay will develop to run predictive assay on marker-trait associations.
This research relates to inhouse objective 2: Develop improved methods of marker-assisted selection and apply markers in development of improved wheat and oat.
Sequence data produced from 454 sequencing of cDNA libraries produced from diverse oat varieties was analyzed for SNP identification. Sequences were used as the bases for development of SNP marker assays using DNA melt curve analyses. Assays were tested on a set of diverse oat lines.
Monitoring: The Principal Investigator attended workshops and meetings of collaborators and conferred with cooperators by phone and by email.