2011 Annual Report 1a.Objectives (from AD-416) This project is a part of the TSTAR-Pacific Program. The purpose of this project is to evaluate the existing germplasm and develop a transformation and selection protocol for salt tolerance swamp taro. The purpose of this project is to allow sustainability to the residents of atolls and low lying areas in Micronesia and American Samoa that are affected by salt water intrusion. 1b.Approach (from AD-416) This a collaborative project with scientist from the American Samoa Community College, College of Micronesia and University of Hawaii to evaluate salt tolerant swamp taro germplasm from American Samoa and Micronesia. The germplasm will be analyzed by molecular techniques and evaluated under high salinity conditions. We will also develop a transformation system and selection procedure to improve salt tolerance of this crop. 3.Progress Report This agreement was established in support of Objective 3 of the in-house project, to develop new knowledge about the genetics, genomics, and transgenics of selected tropical crops. Select lines of Alocasia have been established in tissue culture at American Samoa and will be transferred to Pacific Basin Agricultural Research Center (PBARC) in Hilo. Crytosperma cultivars be currently being collected in the outer atolls of Micronesia and will be sent to PBARC for molecular characterization and will be placed into tissue culture. Salt tolerant Colocasia kalo Pa’akai is being collected in Hawaii and will be tested for salt tolerance to see if this may be a feasible crop for the people in salt inundated region of Micronesia and American Samoa. The lead scientist monitored progress through teleconferences and e-mail.