Cryopreservation and Cryotherapy of Tropical Crops
Plant Germplasm Preservation Research Unit
2011 Annual Report
1a.Objectives (from AD-416)
The objective of this cooperative research is identify methods for increasing tolerance to liquid nitrogen temperatures in avocado and Citrus crops and to determine if pathogens are eliminated during the cryopreservation process.
1b.Approach (from AD-416)
Avocado and Citrus genetic resources will be obtained from the National Plant Germplasm System Repositories in Miami, FL and Riverside, CA, respectively. In vitro cultures will be established and propagated to serve as rootstocks for cryopreserved shoot tips. Cryoprotectant and liquid nitrogen exposure tests will be performed using shoot tips excised from field-grown budwood. Cryopreservation experiments will be performed using disease infected and clean plant materials to determine if liquid nitrogen exposure eradicates diseases from infected tissues.
This project has obtained successfully cryopreserved Citrus shoot tips for seven diverse species. Avocado plants have been established in Fort Collins. Avocado shoot tip growth and proliferation media have been identified. Current research focuses on optimization of cryoprotectant exposure times for improved cryopreservation success in both Citrus and avocado. Periodic collaborator meetings take place as ADODR monitoring activities.