Project Number: 5302-22000-011-02
Project Type: Specific Cooperative Agreement

Start Date: May 18, 2010
End Date: Oct 31, 2012

Objective:
1. Develop qPCR probes for the different Dekopon sub-isolates and quantify each in single and mixed infections to assess interference in accumulation/replication of each sub-isolate. 2. Examine P20, P23, and CP (suppressors of gene silencing) for each sub-isolate for genetic variation and associate with symptom expression. 3. Characterize siRNA profiles in mixed and single infected plants by high throughput sequencing.

Approach:
1. Aphid transmission 2. Reverse transcription (RT) polymerase chain reaction (PCR) 3. Real time PCR (qPCR) 4. Cloning, sequencing 5. Western blots, northern blots, hybridizations 6. Small RNA deep sequencing 7. Bioinformatics Documents SCA with the University of Iowa.