Project Number: 5302-22000-010-04
Project Type: Specific Cooperative Agreement

Start Date: Sep 24, 2010
End Date: May 31, 2015

Objective:
1. Develop metabolic profiles of host responses to “Candidatus Liberibacter” associated diseases. 2. Identify genes involving defensive pathways to pathogen infections.

Approach:
1. Use gas chromatography-mass spectrometry (GC/MS) and high perform liquid chromatography (HPLC) to develop citrus huanglongbing and potato zebra chip metabolic profiles. 2. Use matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS/MS)to identify candidate genes and pathways involving in defensive responses to Liberibacter infections. 3. Identified compounds and amino acid sequences will be compiled by querying the KEGG pathway databases to establish metabolic networks.