Location: Tick and Biting Fly Research
Project Number: 6205-32000-031-23
Start Date: Mar 10, 2010
End Date: Apr 30, 2014
Integrated A. marginale-B. microplus microarrays will be developed using the completed A. marginale genome sequence and the existing B. microplus gene index database of expressed sequence tags. The microarrays will be used to identify networks of pathogen and tick vector gene expression profiles during the development of A. marginale infectivity within B. microplus. Differential gene transcription in the tick will be assayed using midguts and salivary glands of B. microplus, comparing RNA isolated from tissues of ticks reared in conditions under which infectivity develops or fails to develop. Lastly, we will veify the integrated pathogen and tick vector gene expression patterns by proteomic and quantitative PCR analysis.