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United States Department of Agriculture

Agricultural Research Service

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Location: Crop Bioprotection Research

Project Number: 3620-22000-009-00
Project Type: Appropriated

Start Date: Mar 26, 2010
End Date: Apr 20, 2011

Expeditiously identify chemical attractants (e.g., pheromones and plant volatiles) for agriculturally important insect species (either pests or biocontrol agents for weed or insect pests) for which such knowledge is lacking or incomplete, determine the biological and environmental parameters for natural emission of the compounds, and synthesize or otherwise obtain them in quantities sufficient for field use. Characterize the behavioral responses toward the identified compounds under field conditions, with special consideration to the development of practical management tools.

Seek pheromones and host-plant related attractants for selected insect species. Species studied will include, but not be limited to, Diorhabda elongata (a biocontrol agent of saltcedar), Galerucella calmariensis (a biocontrol agent of purple loosestrife), and various flea beetle species (including vegetable crop pests and also biocontrol agents of leafy spurge). Collect volatiles from male and female insects and from host plants and analyze these by gas chromatography, mass spectrometry, and electrophysiology (“electroantennograms”). Those compounds that are emitted by just one sex and that are detected with great sensitivity by the insect antennae are likely to be pheromone components. Similarly, host plant compounds with high antennal sensitivity are potential attractants. Identify the structures of these key compounds using mass spectrometry, nuclear magnetic resonance spectroscopy, chemical tests, and other appropriate methods. Synthesize the compounds using the methods of organic chemistry or otherwise obtain them in bulk from botanical or other sources. Use techniques such as “sticky traps” or other trapping methods to evaluate the attractiveness of synthetic compounds under field conditions, relative to controls and also to live insects. Characterize the behavior toward the attractants under laboratory conditions, if possible. Develop the newly identified attractants as practical insect management tools, for monitoring or manipulating populations of the insects under consideration. For key pheromones, such as those of nitidulid beetles, develop new synthetic schemes suitable for commercial pheromone production. IBC Certified 11/3/10; BSL-1.

Last Modified: 9/20/2014
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