1a.Objectives (from AD-416)
Protect the cellulosic ethanolic fermentations from lactobacilli contaminants that reduce the yield and efficiency of biofuel ethanol production.
1b.Approach (from AD-416)
Identify, subclone, and constitutively express lactobacillus peptidoglycan hydrolase antimicrobials on the surface of fermentative yeast.
There have been three lactobacillus phage endolysins expressed and purified from E. coli expression vectors. One of these is lytic for the target bacterium, L. fermentum and is being tested in three unique expression scenarios designed to express the protein localized to the surface of ethanolic yeast. At this time yeast expression levels are being characterized through a combination of biochemical and antimicrobial assays. This agreement will help improve productivity of ethanolic fermentations, and increase biofuel production. Activity on this project is monitored through monthly conference calls.