1a.Objectives (from AD-416):
To demonstrate that fractionation of biomass to produce an enriched xylan stream is compatible with cellulose conversion into ethanol and, furthermore, with high conversion efficiencies.
1b.Approach (from AD-416):
1. The generated material will be enzymatically hydrolyzed using commercial cellulases and fermented into ethanol using Saccharomyces cerevisiae yeast.
2. A carbohydrate and ethanol mass balance will be set up around the incoming biomass and outgoing ethanol; this balance will be used to solve the process efficiency for conversion of biomass into ethanol.
Hot-water extraction was evaluated as a method for generating xylan oligomers for the perennial grass Miscanthus. Xylan oligomers were recovered using activated carbon followed by extraction in aqueous ethanol solutions. Samples recovered were analyzed for degree of polymerization using high-performance liquid chromatography with pulsed amperometric detector (HPLC-PAD). Data is currently being prepared for publication.