Project Number: 5348-21000-024-15
Start Date: Oct 01, 2010
End Date: Jul 31, 2013
Seed samples will be taken from the advanced pea, lentil and chickpea yield trials planted in the Spring of 2010. Peas and lentils will be grown at Kendrick, ID, Genesee, ID, Pullman, WA and Fairfield, WA. Chickpeas will be grown at Kendrick, ID, Genesee, ID, Pullman, WA and Colton, WA. Each advanced breeding line and commercial check will be grown in three 5 x 20ft2 replicated plots at each location. Samples from winter hardy pea and lentil breeding lines and cultivars will be obtained from advanced winter yield trials planted in the Fall of 2009 at three locations: Pullman, WA; Garfield, WA and Rosalia, WA. A total of 53 advanced pea breeding lines or cultivars, 51 advanced lentil breeding lines or cultivars and 12 advanced chickpea lines or cultivars will be examined. In addition, 13 preliminary pea breeding lines, 15 preliminary lentil lines, and 15 preliminary chickpea lines will be grown at Pullman, WA in three replicated plots and evaluated for protein and mineral content. Plots will be mechanically harvested and yields determined. Seed will be cleaned and 50 g samples will be collected from each plot for determinations of protein and mineral content. To determine the mineral content of each seed sample, seeds (with seed coats) will first be ground to a uniform powder. Two sub-samples from each plot will be wet digested and resuspended using trace metal-grade nitric acid and hydrogen peroxide. Two replicate digests will be analyzed for mineral concentration using inductively-coupled plasma atomic-emission spectroscopy (ICP-OES). This technique will provide analysis of K, P, Ca, Mg, Fe, Mn, Zn, Cu, Ni, and Mo. Seed protein concentrations will be derived based on seed nitrogen concentrations, which will be established using a LECO FP-528 Nitrogen/Protein Determinator.