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United States Department of Agriculture

Agricultural Research Service

Research Project: Application of Molecular Technologies to Improve Conventional Breeding Efficiencies in Peanut and Warm Season Grasses

Location: Crop Genetics and Breeding Research

2011 Annual Report

1a.Objectives (from AD-416)
Application of marker assisted selection to improve the efficiencies of breeding peanut and warm season grasses.

1b.Approach (from AD-416)
Segregating populations of peanut will be developed, grown, and analyzed for molecular markers. Data will be used to select genotypes with improved disease resistance and improved quality traits.

Segregating populations of warm season grasses will be developed, grown, and analyzed for molecular markers. Data will be used to select improved genotypes for turf, bioenergy, and other uses.

3.Progress Report

This research relates to the inhouse objective 2: Development of peanut germplasm with high oleic acid and resistance to the peanut root-knot nematode and tomato spotted wilt virus.

Several thousand plants from segregating populations were sampled and deoxyribose nucleic acid (DNA)was extracted. DNA was then analyzed to identify segregants containing alleles for high oleic and nematode resistance, and these results were provided to ARS scientist to aid in our breeding selection process. Molecular markers were also used to evaluate the level of genetic polymorphism in several other segregating populations which should aid in the development of markers for quantitative trait loci (QTLs) that can be used to improve future breeding efficiencies.

The Authorized Departmental Officer's Designated Representative for this project has monitored project progress through progress reports and through regular email contact with project collaborators.

Last Modified: 7/1/2015
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